Alternatives Searching and Strategies for Animal Research Protocols

Step 1: Define the Question

Formulating the Question

It may help to think of your research question in four parts. Leenaars, et al. suggest four components for a focused research question in the field of animal research:

   1.) Intervention/Exposure

   2.) Disease of interest/health problem

   3.) Animal/animal species/population

   4.) Outcome measures

A research question including these concepts could be formatted as "What is the effect of [intervention/exposure] on [outcome measures] in [animal species/population] for [disease/health problem]?"

Brainstorming Keywords

It may be useful to first determine what keywords specific to your research protocol are necessary, and then to add keywords for the reduction, refinement, or replacement of animal research subjects (the "Three R's").

   Tips for Research-Specific Keywords

  • Scientific and common names of any species being studied
  • Scientific and common names of any diseases or conditions being studied
  • Generic name, trade names, and CAS RN of drugs and/or chemicals being used
  • Treatments being used
  • Scientific and common terms for tissues, systems, or cell lines being studied


Leenaars, M., Hooijmans, C., van Veggel, N., ter Riet, G., Leeflang, M., Hooft, L., van der Wilt, G.J., Tillema, A., and Ritskes-Hoitinga, M. (2012). A step-by-step guide to systematically identify all relevant animal studies. Laboratory Animals, 46(1), 24-31. doi:10.1258/la.2011.011087